Semi-micro solid phase extraction with electrospun polystyrene fiber disks

Samuel Chigome, Godfred Darko, Ulrich Buttner, Nelson Torto*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

A technique is described for performing solid phase extraction (SPE) at a semi-microscale. 10 mg of electrospun polystyrene fibers (average diameter 2.7 m) were packed into a SPE barrel to form a disk (5 mm × 1 mm). The device was employed to evaluate the extraction of four steroids: prednisone, hydrocortisone, cortisone acetate and 19-nortestosterone from water and plasma matrices. The analytes were desorbed from the fibers with 100 l methanol and monitored by high performance liquid chromatography with a diode array detector (HPLC-DAD). The semi-micro SPE method provided extraction recoveries of 51.14-80.13% in plasma and 66.07-93.43% in water. The breakthrough volumes at 500 ng ml-1 ranged from 200-400 l for all analytes. At optimal conditions, the four analytes followed an excellent linear relationship in the range 12.5-400 ng ml-1 with coefficients of determination (r 2) greater than 0.99 and the limits of detection ranged from 0.75 to 1.29 ng ml-1. Due to its simplicity, it is anticipated that the method will greatly simplify disk solid phase extraction.

Original languageEnglish (US)
Pages (from-to)623-626
Number of pages4
JournalAnalytical Methods
Volume2
Issue number6
DOIs
StatePublished - Jun 2010
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Engineering(all)
  • Chemical Engineering(all)

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