Second harmonic phase microscopy of collagen fibers

Etienne Shaffer*, Pierre Marquet, Christian Depeursinge

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

In the past decade, quantitative phase imaging gave a new dimension to optical microscopy, and the recent extension of digital holography techniques to nonlinear microscopy appears very promising, for the phase of nonlinear signal provides additional information, inaccessible to incoherent imaging schemes. Last year, we have reported how the SHG phase makes possible real-time nanometric 3D-tracking of SHG emitters, such as nanoparticles (Shaffer et al., Opt. Express 18, p.17392-17403, 2010). Here, we investigate the phase of second harmonic generated by a label-free biological specimen-more precisely collagen fibers forming the connective tissue of a mouse dermis- and discuss of its interpretation. Notably, we show how the SHG phase, qualitatively acting as an indicator of phase-matching conditions, tends to indicate that second harmonic generation, in collagen, is dominated by coherent SHG scattering.

Original languageEnglish (US)
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XI
DOIs
StatePublished - May 5 2011
EventMultiphoton Microscopy in the Biomedical Sciences XI - San Francisco, CA, United States
Duration: Jan 23 2011Jan 25 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7903
ISSN (Print)1605-7422

Other

OtherMultiphoton Microscopy in the Biomedical Sciences XI
CountryUnited States
CitySan Francisco, CA
Period01/23/1101/25/11

Keywords

  • Digital holographic microscopy (DHM)
  • Holographic SHG imaging
  • Multiphoton microscopy
  • Nonlinear phase microscopy
  • Second harmonic generation (SHG)

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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