Saponaria officinalis karyology and karyotype by means of image analyzer and atomic force microscopy

S. Di Bucchianico, G. Venora, S. Lucretti, T. Limongi, L. Palladino, A. Poma*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The aim of this work was to offer a contribution to the characterization of taxonomic entity of Saponaria officinalis (2n 5 28; an herbaceous perennial species; saporin, a type 1 Ribosome Inactivating Protein, is present in leaves and seeds) by a cytogenetic and karyomorphological approach. We investigated the karyotype's morphometry correlated with Stebbin's symmetric index; the same information has been used for computing the indices resemblance between chromosomes (REC), symmetric indices (SYI), and total form (TF%) which allow the comparison between species and evaluation of karyological evolution. Fluorescence intensities of the stained nuclei were measured by a flow cytometer and, for the first time, values for nuclear DNA content were estimated by comparing nuclei fluorescence intensities of the test population with those of appropriate internal DNA standards. Our study is also aimed to introduce chromosomal volumes, which were determined by atomic force microscopy (AFM), as novel karyomorphological parameter which could allow for chromosome discrimination especially when tiny ones are present.

Original languageEnglish (US)
Pages (from-to)730-736
Number of pages7
JournalMicroscopy Research and Technique
Volume71
Issue number10
DOIs
StatePublished - Oct 2008
Externally publishedYes

Keywords

  • Atomic force microscopy
  • Chromosomal volumes
  • Flow cytometry
  • Karyology
  • Karyotype
  • Saponaria officinalis

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Instrumentation
  • Medical Laboratory Technology

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