Salt stress induces changes in amounts and localization of the mitogen-activated protein kinase SIMK in alfalfa roots

František Baluška, Miroslav Ovecka, Heribert Hirt*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

SIMK is an alfalfa mitogen-activated protein kinase (MAPK) that is activated by salt stress and shows a nuclear localization in suspension-cultured cells. We investigated the localization of SIMK in alfalfa (Medicago sati a) roots. Although SIMK was expressed in most tissues of the root apex, cells of the quiescent center and statocytes showed much lower SIMK protein amounts. In cells of the elongation zone, SIMK was present in much higher amounts in epidermal than in cortex cells. In dividing cells of the root tip, SIMK revealed a cell cycle phase-dependent localization, being predominantly nuclear in interphase but associating with the cell plate and the newly formed cell wall in telophase and early G 1 phase. In dividing cells, salt stress resulted in an association of part of the SIMK with the preprophase band. Generally, salt stress resulted in much higher amounts of SIMK in dividing cells of the root apex and epidermal cells of the elongation zone. These data demonstrate that amounts and subcellular localization of SIMK in roots is highly regulated and sensitive to environmental stress.

Original languageEnglish (US)
Pages (from-to)262-267
Number of pages6
JournalProtoplasma
Volume212
Issue number3-4
StatePublished - Jan 1 2000

Keywords

  • Medicago sati a
  • Mitogen-activated protein kinase
  • Osmotic stress
  • Salt stress

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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