Salmonella enterica flagellin is recognized via FLS2 and activates PAMP-triggered immunity in Arabidopsis thaliana

Ana Victoria Garcia*, Amélie Charrier, Adam Schikora, Jean Bigeard, Stephanie Pateyron, Marie Ludivine De Tauzia-Moreau, Alexandre Evrard, Axel Mithöfer, Marie Laure Martin-Magniette, Isabelle Virlogeux-Payant, Heribert Hirt

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Infections with Salmonella enterica belong to the most prominent causes of food poisoning and infected fruits and vegetables represent important vectors for salmonellosis. Recent evidence indicates that plants recognize S. enterica and raise defense responses. Nonetheless, the molecular mechanisms controlling the interaction of S. enterica with plants are still largely unclear. Here, we show that flagellin from S. enterica represents a prominent pathogen-associated molecular pattern (PAMP) in Arabidopsis thaliana, which induces PAMP-triggered immunity (PTI) via the recognition of the flg22 domain by the receptor kinase FLS2. The Arabidopsis fls2 mutant shows reduced though not abolished PTI activation, indicating that plants rely also on recognition of other S. enterica PAMPs. Interestingly, the S. enterica type III secretion system (T3SS) mutant prgH- induced stronger defense gene expression than wild-type bacteria in Arabidopsis, suggesting that T3SS effectors are involved in defense suppression. Furthermore, we observe that S. enterica strains show variation in the flg22 epitope, which results in proteins with reduced PTI-inducing activity. Altogether, these results show that S. enterica activates PTI in Arabidopsis and suggest that, in order to accomplish plant colonization, S. enterica evolved strategies to avoid or suppress PTI.

Original languageEnglish (US)
Pages (from-to)657-674
Number of pages18
JournalMolecular Plant
Volume7
Issue number4
DOIs
StatePublished - Jan 1 2014

Keywords

  • Arabidopsis thaliana
  • FLS2
  • Salmonella enterica
  • flagellin
  • immunity.

ASJC Scopus subject areas

  • Molecular Biology
  • Plant Science

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