Root stem cell niche organizer specification by molecular convergence of PLETHORA and SCARECROW transcription factor modules

Akie Shimotohno, Renze Heidstra, Ikram Blilou, Ben Scheres*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Continuous formation of somatic tissues in plants requires functional stem cell niches where undifferentiated cells are maintained. In Arabidopsis thaliana, PLETHORA (PLT) and SCARECROW (SCR) genes are outputs of apical– basal and radial patterning systems, and both are required for root stem cell specification and maintenance. The WUSCHEL-RELATED HOMEOBOX 5 (WOX5) gene is specifically expressed in and required for functions of a small group of root stem cell organizer cells, also called the quiescent center (QC). PLT and SCR are required for QC function, and their expression overlaps in the QC; however, how they specify the organizer has remained unknown. We show that PLT and SCR genetically and physically interact with plant-specific teosinte-branched cycloidea PCNA (TCP) transcription factors to specify the stem cell niche during embryogenesis and maintain organizer cells post-embryonically. PLT–TCP–SCR complexes converge on PLT-binding sites in the WOX5 promoter to induce expression.

Original languageEnglish (US)
Pages (from-to)1085-1100
Number of pages16
JournalGenes and Development
Volume32
Issue number15-16
DOIs
StatePublished - Aug 1 2018

Keywords

  • Arabidopsis
  • Organizer
  • PLT-TCP-SCR complexes
  • Quiescent center
  • Stem cell niche
  • WUSCHEL-RELATED HOMEOBOX 5

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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