RNA-Binding Proteins Revisited – The Emerging Arabidopsis mRNA Interactome

Tino Köster, Claudius Marondedze, Katja Meyer, Dorothee Staiger

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

RNA–protein interaction is an important checkpoint to tune gene expression at the RNA level. Global identification of proteins binding in vivo to mRNA has been possible through interactome capture – where proteins are fixed to target RNAs by UV crosslinking and purified through affinity capture of polyadenylated RNA. In Arabidopsis over 500 RNA-binding proteins (RBPs) enriched in UV-crosslinked samples have been identified. As in mammals and yeast, the mRNA interactomes came with a few surprises. For example, a plethora of the proteins caught on RNA had not previously been linked to RNA-mediated processes, for example proteins of intermediary metabolism. Thus, the studies provide unprecedented insights into the composition of the mRNA interactome, highlighting the complexity of RNA-mediated processes.
Original languageEnglish (US)
Pages (from-to)512-526
Number of pages15
JournalTrends in Plant Science
Volume22
Issue number6
DOIs
StatePublished - Apr 13 2017
Externally publishedYes

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