Relationships between serotypes and genotypes of hepatitis B virus: genetic classification of HBV by use of surface genes

Ken ichi Ohba, Masashi Mizokami*, Tomoyoshi Ohno, Kaoru Suzuki, Etsuro Orito, Johnson Y.N. Lau, Yasuo Ina, Kazuho Ikeo, Takashi Gojobori

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Hepatitis B virus (HBV) surface antigen (HBsAg), which is encoded by the HBV S gene, is conventionally classified into 4 serological subtypes, adw, adr, ayw and ayr. To determine the relationship between the HBsAg seroreactivity and the nucleotide sequence diversity of the HBV S gene, the nucleotide sequences of S genes for HBV isolates reported so far were aligned with each other. The numbers of nucleotide substitutions were then estimated by the 6-parameter method, and a phylogenetic tree was constructed by the unweighted paired grouping method with arithmetic mean (UPGMA) and the neighbor-joining (NJ) method. The phylogenetic trees constructed showed that all isolates were grouped into 4 genotypes (gyw, gdw-1, gdw-2, and gdr). More importantly, the genotypes did not necessarily correspond to the conventional serotypes. In particular, serotype 'adw' can be any of genotypes gdw-1, gdw-2, or gdr. Thus, genotyping by S genes gives more accurate information about genetic variation of HBV.

Original languageEnglish (US)
Pages (from-to)25-34
Number of pages10
JournalVirus Research
Volume39
Issue number1
DOIs
StatePublished - Jan 1 1995

Keywords

  • Genotype
  • Hepatitis B virus (HBV)
  • Molecular evolution
  • Phylogenetic tree
  • Subtype

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

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