Regulation of the heat stress response in arabidopsis by MPK6-targeted phosphorylation of the heat stress factor HsfA2

Alexandre Evrard, Mukesh Kumar, David Lecourieux, Jessica Lucks, Pascal von Koskull-Döring, Heribert Hirt*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

So far little is known on the functional role of phosphorylation in the heat stress response of plants. Here we present evidence that heat stress activates the Arabidopsis mitogen-activated protein kinase MPK6. In vitro and in vivo evidence is provided that MPK6 specifically targets the major heat stress transcription factor HsfA2. Activation of MPK6 results in complex formation with HsfA2. MPK6 phosphory- lates HsfA2 on T249 and changes its intracellular localisation. Protein kinase and phosphatase inhibitor studies indicate that HsfA2 protein stability is regulated in a phosphorylation-dependent manner, but this mechanism is independent of MPK6. Overall, our data show that heat stress-induced targeting of HsfA2 by MPK6 partici- pates in the complex regulatory mechanism how plants respond to heat stress.

Original languageEnglish (US)
Article numbere59
JournalPeerJ
Volume2013
Issue number1
DOIs
StatePublished - May 10 2013

Keywords

  • Arabidopsis
  • Heat stress proteins
  • Heat stress transcription factors
  • Kinases
  • MAP kinase

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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