Regenerant arabidopsis lineages display a distinct genome-wide spectrum of mutations conferring variant phenotypes

Caifu Jiang, Aziz Mithani, Xiangchao Gan, Eric J. Belfield, John Klingler, Jian-Kang Zhu, Jiannis Ragoussis, Richard Mott, Nicholas P. Harberd

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

Multicellular organisms can be regenerated from totipotent differentiated somatic cell or nuclear founders [1-3]. Organisms regenerated from clonally related isogenic founders might a priori have been expected to be phenotypically invariant. However, clonal regenerant animals display variant phenotypes caused by defective epigenetic reprogramming of gene expression [2], and clonal regenerant plants exhibit poorly understood heritable phenotypic ("somaclonal") variation [4-7]. Here we show that somaclonal variation in regenerant Arabidopsis lineages is associated with genome-wide elevation in DNA sequence mutation rate. We also show that regenerant mutations comprise a distinctive molecular spectrum of base substitutions, insertions, and deletions that probably results from decreased DNA repair fidelity. Finally, we show that while regenerant base substitutions are a likely major genetic cause of the somaclonal variation of regenerant Arabidopsis lineages, transposon movement is unlikely to contribute substantially to that variation. We conclude that the phenotypic variation of regenerant plants, unlike that of regenerant animals, is substantially due to DNA sequence mutation. 2011 Elsevier Ltd. All rights reserved.
Original languageEnglish (US)
Pages (from-to)1385-1390
Number of pages6
JournalCurrent Biology
Volume21
Issue number16
DOIs
StatePublished - Aug 2 2011

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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