Reflection-mode TERS on Insulin Amyloid Fibrils with Top-Visual AFM Probes

Manola Moretti*, Remo Proietti Zaccaria, Emiliano Descrovi, Gobind Das, Marco Leoncini, Carlo Liberale, Francesco de Angelis, Enzo Di Fabrizio

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Tip-enhanced Raman spectroscopy provides chemical information while raster scanning samples with topographical detail. The coupling of atomic force microscopy and Raman spectroscopy in top illumination optical setup is a powerful configuration to resolve nanometer structures while collecting reflection mode backscattered signal. Here, we theoretically calculate the field enhancement generated by TER spectroscopy with top illumination geometry and we apply the technique to the characterization of insulin amyloid fibrils. We experimentally confirm that this technique is able to enhance the Raman signal of the polypeptide chain by a factor of 105, thus revealing details down to few molecules resolution.

Original languageEnglish (US)
Pages (from-to)25-33
Number of pages9
Issue number1
StatePublished - Mar 19 2013


  • Amyloid fibrils
  • Field enhancement
  • Raman spectroscopy
  • TERS
  • Top-illumination setup

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biochemistry


Dive into the research topics of 'Reflection-mode TERS on Insulin Amyloid Fibrils with Top-Visual AFM Probes'. Together they form a unique fingerprint.

Cite this