Real-time single-molecule observation of rolling-circle DNA replication

Nathan A. Tanner, Joseph J. Loparo, Samir Hamdan, Slobodan Jergic, Nicholas E. Dixon, Antoine M. van Oijen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

We present a simple technique for visualizing replication of individual DNA molecules in real time. By attaching a rolling-circle substrate to a TIRF microscope-mounted flow chamber, we are able to monitor the progression of single-DNA synthesis events and accurately measure rates and processivities of single T7 and Escherichia coli replisomes as they replicate DNA. This method allows for rapid and precise characterization of the kinetics of DNA synthesis and the effects of replication inhibitors.

Original languageEnglish (US)
Article numbere27
JournalNucleic acids research
Volume37
Issue number4
DOIs
StatePublished - Mar 24 2009

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Real-time single-molecule observation of rolling-circle DNA replication'. Together they form a unique fingerprint.

Cite this