Real-time agglutination within a microdroplet in a three phase fluidic well for detection of biomarkers

Shilpa Sivashankar, Daniela Castro, Ulrich Buttner, Ian Grant Foulds

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

4 Scopus citations

Abstract

In the proposed paper, we describe a quantitative detection method that avoids the hook-effect which we call real-time agglutination. Agglutination is monitored optically in a droplet containing functionalized beads suspended at the interface of two immiscible fluids. Analyte is then added slowly through a capillary. This slow addition of analyte allows the avoidance of the hook-effect because strong agglutination occurs prior bead saturation. Analyte concentration in the sample can then be extracted from the time vs. agglutination strength curve, which shows relationship to analyte concentration in both onset and rate of agglutination. Real-time agglutination can find use as a biomolecule quantification method as well as a test bed to look at agglutination kinetics.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages2097-2100
Number of pages4
ISBN (Electronic)9780979806476
StatePublished - Jan 1 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Keywords

  • Agglutination assay
  • Hook-effect
  • Micro droplets

ASJC Scopus subject areas

  • Control and Systems Engineering

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