Rapid reversed-phase separation of proteins and peptides using optimized 'moulded' monolithic poly(styrene-co-divinylbenzene) columns

Shaofeng Xie*, Robert W. Allington, Frantisek Svec, Jean Frechet

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

113 Scopus citations

Abstract

Monolithic macroporous poly(styrene-co-divinylbenzene) stationary phases have been prepared by free radical polymerization within the confines of 4.6-mm I.D. chromatographic columns. The optimized porous properties allow the mobile phase to flow through these columns at flow-rates of up to 10 ml/min. As opposed to the simultaneously tested columns packed with either silica or synthetic polymer beads, the monoliths exhibit only modest back pressure. The monolithic columns were able to separate mixtures of peptides and proteins in a very short time. Under the optimized conditions, the separation of five proteins can be easily achieved in less than 20 s. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)169-174
Number of pages6
JournalJournal of Chromatography A
Volume865
Issue number1-2
DOIs
StatePublished - Dec 31 1999

Keywords

  • LC
  • Peptides
  • Poly(styrene-divinylbenzene) stationary phases
  • Proteins
  • Stationary phases

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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