Rapid identification of rust resistance genes through cultivar-specific de novo chromosome assemblies

Anupriya K. Thind, Thomas Wicker, Simon Krattinger*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

“Map-based cloning” is a frequently used approach to isolate rust resistance genes. A critical step during map-based cloning is the transition from genetic information, i.e., a genetic map, to physical sequence information. Bacterial artificial chromosome clones are often used to establish sequence information spanning a genetic interval. However, a major limitation of BAC clones consists in their small insert size of 100–200 kb. Targeted chromosome-based cloning via long-range assembly (TACCA) is a method that can replace BAC library screening. This approach involves chromosome flow-sorting and the establishment of a long-range de novo assembly. This chapter provides an overview of TACCA as well as a detailed description of sequence analyses, molecular marker development, and candidate gene identification.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages245-255
Number of pages11
DOIs
StatePublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1659
ISSN (Print)1064-3745

Keywords

  • Candidate gene
  • Chromosome flow-sorting
  • Long-range scaffolding
  • Map-based cloning
  • Molecular marker

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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