Abstract
Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics.
Original language | English (US) |
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Article number | 221 |
Journal | Genome biology |
Volume | 17 |
Issue number | 1 |
DOIs | |
State | Published - Oct 31 2016 |
Keywords
- Barley
- Chromosome flow sorting
- Gene cloning
- MutChromSeq
- Mutational genomics
- Triticeae
- Wheat
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Genetics
- Cell Biology