Rapid gene isolation in barley and wheat by mutant chromosome sequencing

Javier Sánchez-Martín, Burkhard Steuernagel, Sreya Ghosh, Gerhard Herren, Severine Hurni, Nikolai Adamski, Jan Vrána, Marie Kubaláková, Simon Krattinger, Thomas Wicker, Jaroslav Doležel, Beat Keller*, Brande B.H. Wulff

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

107 Scopus citations

Abstract

Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics.

Original languageEnglish (US)
Article number221
JournalGenome biology
Volume17
Issue number1
DOIs
StatePublished - Oct 31 2016

Keywords

  • Barley
  • Chromosome flow sorting
  • Gene cloning
  • MutChromSeq
  • Mutational genomics
  • Triticeae
  • Wheat

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Rapid gene isolation in barley and wheat by mutant chromosome sequencing'. Together they form a unique fingerprint.

Cite this