Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon

Christian Frøkjær-Jensen*, M. Wayne Davis, Mihail Sarov, Jon Taylor, Stephane Flibotte, Matthew LaBella, Andrei Pozniakovsky, Donald G. Moerman, Erik M. Jorgensen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

143 Scopus citations

Abstract

We have generated a recombinant Mos1 transposon that can insert up to 45-kb transgenes into the Caenorhabditis elegans genome. The minimal Mos1 transposon (miniMos) is 550 bp long and inserts DNA into the genome at high frequency (~60% of injected animals). Genetic and antibiotic markers can be used for selection, and the transposon is active in C. elegans isolates and Caenorhabditis briggsae. We used the miniMos transposon to generate six universal Mos1-mediated single-copy insertion (mosSCI) landing sites that allow targeted transgene insertion with a single targeting vector into permissive expression sites on all autosomes. We also generated two collections of strains: a set of bright fluorescent insertions that are useful as dominant, genetic balancers and a set of lacO insertions to track genome position.

Original languageEnglish (US)
Pages (from-to)529-534
Number of pages6
JournalNature Methods
Volume11
Issue number5
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon'. Together they form a unique fingerprint.

Cite this