Quantitative volumetric Raman imaging of three dimensional cell cultures

Charalambos Kallepitis, Mads S. Bergholt, Manuel M. Mazo, Vincent Leonardo, Stacey C. Skaalure, Stephanie A. Maynard, Molly M. Stevens

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

The ability to simultaneously image multiple biomolecules in biologically relevant three-dimensional (3D) cell culture environments would contribute greatly to the understanding of complex cellular mechanisms and cell–material interactions. Here, we present a computational framework for label-free quantitative volumetric Raman imaging (qVRI). We apply qVRI to a selection of biological systems: human pluripotent stem cells with their cardiac derivatives, monocytes and monocyte-derived macrophages in conventional cell culture systems and mesenchymal stem cells inside biomimetic hydrogels that supplied a 3D cell culture environment. We demonstrate visualization and quantification of fine details in cell shape, cytoplasm, nucleus, lipid bodies and cytoskeletal structures in 3D with unprecedented biomolecular specificity for vibrational microspectroscopy.
Original languageEnglish (US)
JournalNature Communications
Volume8
Issue number1
DOIs
StatePublished - Mar 22 2017
Externally publishedYes

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