Quantitative proteomics reveals differential biological processes in healthy neonatal cord neutrophils and adult neutrophils

Jiang Zhu, Huoming Zhang, Tiannan Guo, Wenying Li, Huiyu Li, Yi Zhu, Shiang Huang

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Neonatal neutrophils are characterized by the immaturity of bactericidal mechanisms that contributes largely to neonatal mortality. However, underlying molecular mechanism associated with the immaturity remains incompletely understood. In this study, we performed comparative proteomic analysis on neonatal neutrophils derived from human cord blood and adult peripheral neutrophils. A total of 1332 proteins were identified and quantified, and 127 proteins were characterized as differentially expressed between adult and cord neutrophils. The differentially expressed proteins are mapped in KEGG pathways into five clusters and indicated impaired functions of neonatal neutrophils in proteasome, lysosome, phagosome, and leukocyte transendothelial migration. In particular, many proteins associated with NETosis, a critical mechanism for antimicrobial process and auto-clearance, were also found to be downregulated in cord neutrophils. This study represents a first comparative proteome profiling of neonatal and adult neutrophils, and provides a global view of differentially expressed proteome for enhancing our understanding of their various functional difference. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Original languageEnglish (US)
Pages (from-to)1688-1697
Number of pages10
JournalPROTEOMICS
Volume14
Issue number13-14
DOIs
StatePublished - Jun 11 2014

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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