Quantitative Phosphoproteomic Using Titanium Dioxide Micro-Columns and Label-Free Quantitation

Martin Barrios-Llerena, Thierry Le Bihan

    Research output: Chapter in Book/Report/Conference proceedingChapter

    1 Scopus citations

    Abstract

    Phosphorylation events are important during cellular function. Analysis of phosphorylation in complex samples has been extensively studied using large-scale phosphopeptide enrichment methods. Quantitative analysis of the enriched phosphopeptides is subsequently performed using label-based methodologies (e.g., SILAC, iTRAQ, and others). Here we describe the protocol for the quantitative analysis of phosphopeptides, enriched with titanium dioxide micro-column, using an intensity-based label-free quantitation.
    Original languageEnglish (US)
    Title of host publicationMass Spectrometry of Proteins
    PublisherSpringer Nature
    Pages35-42
    Number of pages8
    ISBN (Print)9781493992317
    DOIs
    StatePublished - Apr 13 2019

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