Quantitative phase contrast microscopy of living cells by numerical reconstruction of digital holograms

Etienne Cuche*, Pierre Marquet, Pierre J. Magistretti, Christian Depeursinge

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

7 Scopus citations

Abstract

We describe a new method of optical microscopy which allows simultaneously intensity and quantitative phase contrast imaging. The method is based on a new holographic imaging technique which involves a CCD camera as recording device and a numerical method for the reconstruction. An application to phase contrast imaging of living astrocytes is presented.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages84-89
Number of pages6
Volume3604
ISBN (Print)0819430749
StatePublished - 1999
Externally publishedYes
EventProceedings of the 1999 Optical Diagnostics of Living cells - San Jose, CA, USA
Duration: Jan 25 1999Jan 26 1999

Other

OtherProceedings of the 1999 Optical Diagnostics of Living cells
CitySan Jose, CA, USA
Period01/25/9901/26/99

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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