Provitamin a accumulation in cassava (Manihot esculenta) roots driven by a single nucleotide polymorphism in a phytoene synthase gene

Ralf Welsch, Jacobo Arango, Cornelia Bär, Bertha Salazar, Salim Al-Babili, Jesús Beltrán, Paul Chavarriaga, Hernan Ceballos, Joe Tohme, Peter Beyera*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

156 Scopus citations

Abstract

Cassava (Manihot esculenta) is an important staple crop, especially in the arid tropics. Because roots of commercial cassava cultivars contain a limited amount of provitamin A carotenoids, both conventional breeding and genetic modification are being applied to increase their production and accumulation to fight vitamin A deficiency disorders. We show here that an allelic polymorphism in one of the two expressed phytoene synthase (PSY) genes is capable of enhancing the flux of carbon through carotenogenesis, thus leading to the accumulation of colored pr ovitamin A carotenoids instorage roots. A single nucleotide polymorphism present only in yellow-rooted cultivars cosegregates with colored roots in a breeding pedigree. The resulting amino acid exchange in a highly conserved region of PSY provides increased catalytic activity in vitro and is able to increase carotenoid production in recombinant yeast and Escherichia coli cells. Consequently, cassava plants over expressing a PSY transgene produce yellow-fleshed, high-carotenoid roots. This newly characterizedPSY allele provides means to improve cassava provitamin A content in cassava roots through both breeding and genetic modification.

Original languageEnglish (US)
Pages (from-to)3348-3356
Number of pages9
JournalPlant Cell
Volume22
Issue number10
DOIs
StatePublished - Oct 1 2010

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'Provitamin a accumulation in cassava (Manihot esculenta) roots driven by a single nucleotide polymorphism in a phytoene synthase gene'. Together they form a unique fingerprint.

Cite this