Proteomic Investigation of Rhizoctonia solani AG 4 Identifies Secretome and Mycelial Proteins with roles in Plant Cell Wall Degradation and Virulence

Dilip Lakshman, Daniel P. Roberts, Wesley M. Garrett, Savithiry S. Natarajan, Omar Darwish, Nadim Alkharouf, Arnab Pain, Farooq Khan, Prashant P. Jambhulkar, Amitava Mitra

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Rhizoctonia solani AG 4 is a soilborne necrotrophic fungal plant pathogen that causes economically important diseases on agronomic crops worldwide. Here we used a proteomics approach to characterize both intracellular proteins and the secretome of R. solani AG 4 isolate Rs23A under several growth conditions; the secretome being highly important in pathogenesis. From over 500 total secretome and soluble intracellular protein spots from 2-D gels, 457 protein spots were analyzed and 318 proteins positively matched with fungal proteins of known function by comparison with available R. solani genome databases specific for anastomosis groups 1-IA, 1-IB, and 3. These proteins were categorized to possible cellular locations and functional groups; and for some proteins their putative roles in plant cell wall degradation and virulence. The majority of the secreted proteins were grouped to extracellular regions and contain hydrolase activity.
Original languageEnglish (US)
Pages (from-to)3101-3110
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume64
Issue number15
DOIs
StatePublished - Apr 6 2016

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