Properties of the force exerted by filopodia and lamellipodia and the involvement of cytoskeletal components

Dan Cojoc*, Francesco Difato, Enrico Ferrari, Rajesh B. Shahapure, Jummi Laishram, Massimo Righi, Enzo Di Fabrizio, Vincent Torre

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

93 Scopus citations

Abstract

During neuronal differentiation, lamellipodia and filopodia explore the environment in search for the correct path to the axon's final destination. Although the motion of lamellipodia and filopodia has been characterized to an extent, little is knowip about the force they exert. In this study, we used optical tweezers to measure the force exerted by filopodia and lamellipodia with a millisecond temporal resolution. We found that a single filopodium exerts a force not exceeding 3 pN, whereas lamellipodia can exert a force up to 20 pN. Using metabolic inhibitors, we showed that no force is produced in the absence of actin polymerization and that development of forces larger than 3 pN requires microtubule polymerization. These results show that actin, polymerization is necessary for for production and demonstrate that not only do neurons process information, but they also act on their environment exerting forces varying from tenths pN to tens of pN.

Original languageEnglish (US)
Article numbere1072
JournalPloS one
Volume2
Issue number10
DOIs
StatePublished - Oct 24 2007

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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