Progress and perspectives in digital holographic microscopy applied to life sciences

Pierre Marquet*, Jonas Kühn, Daniel Boss, Pascal Jourdain, Pierre Magistretti, Nicoals Pavillon, Christian Depeursinge

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. The quantitative phase images allow the derivation of highly relevant cell parameters, including dry mass density and spatial distribution. Thanks to a decoupling procedure, cell thickness and intracellular refractive index can be measured separately. Consequently, cell morphology, shape as well as cell membrane fluctuations can be accurately monitor. As far as red blood cell are considered, Mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), two highly relevant clinical parameters, have been measured non-invasively at a single cell level. The DHM nanometric axial and microsecond temporal sensitivities have permitted to measure the red blood cell membrane fluctuations (CMF) over the whole cell surface. In addition, the development of live-cell multimodality microscope combining fluorescence with digital holographic microscopy is presented. The biophysical cell parameters derived from the quantitative phase information in combination with the numerous different specific fluorescent cellular probes allow to this multimodality microscope to address various important issues in cell biology.

Original languageEnglish (US)
Title of host publication2010 9th Euro-American Workshop on Information Optics, WIO 2010
StatePublished - 2010
Externally publishedYes
Event2010 9th Euro-American Workshop on Information Optics, WIO 2010 - Helsinki, Finland
Duration: Jul 12 2010Jul 16 2010


Other2010 9th Euro-American Workshop on Information Optics, WIO 2010

ASJC Scopus subject areas

  • Information Systems
  • Atomic and Molecular Physics, and Optics

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