Profiling of Root Canal Bacterial Communities Associated with Chronic Apical Periodontitis from Brazilian and Norwegian Subjects

José F. Siqueira, Isabela N. Rôças, Gilberto J. Debelian, Flávia L. Carmo, Simone S.M. Paiva, Flávio R.F. Alves, Alexandre S. Rosado

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

The aim of this study was to compare the bacterial community profiles of the root canal microbiota associated with chronic apical periodontitis from Brazilian and Norwegian patients using the denaturing gradient gel electrophoresis (DGGE) and the ribosomal intergenic spacer analysis (RISA) approaches. DNA extracted from root canal samples was subjected to polymerase chain reaction using primers appropriate for further DGGE or RISA analysis. The resulting banding patterns representative of the bacterial community structures in samples from the two locations were compared. DGGE and RISA fingerprints showed a great interindividual variability in the bacterial community profiles, irrespective of the geographic location of the patient. However, similarities among the bacterial community DGGE profiles revealed the existence of a geography-related pattern. © 2008 American Association of Endodontists.
Original languageEnglish (US)
Pages (from-to)1457-1461
Number of pages5
JournalJournal of Endodontics
Volume34
Issue number12
DOIs
StatePublished - Dec 1 2008
Externally publishedYes

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