Prediction of functionally important sites from protein sequences using sparse kernel least squares classifiers

Ke Tang, Pugalenthi Ganesan, P. N. Suganthan, Christopher J. Lanczycki, Saikat Chakrabarti*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    6 Scopus citations

    Abstract

    Identification of functionally important sites (FIS) in proteins is a critical problem and can have profound importance where protein structural information is limited. Machine learning techniques have been very useful in successful classification of many important biological problems. In this paper, we adopt the sparse kernel least squares classifiers (SKLSC) approach for classification and/or prediction of FIS using protein sequence derived features. The SKLSC algorithm was applied to 5435 FIS that have been extracted from 312 reliable alignments for a wide range of protein families. We obtained 68.28% sensitivity and 68.66% specificity for training dataset and 65.34% sensitivity and 66.88% specificity for testing dataset. Further, large scale benchmarking study using alignments of 101 protein families containing 1899 FIS showed that our method achieved an average ∼70% sensitivity in predicting different types of FIS, such as active sites, metal, ligand or protein binding sites. Our findings also indicate that active sites and metal binding sites are comparably easier to predict compared to the ligand and protein binding sites. Despite moderate success, our results suggest the usefulness and potential of SKLSC approach in prediction of FIS using only protein sequence derived information.

    Original languageEnglish (US)
    Pages (from-to)155-159
    Number of pages5
    JournalBiochemical and Biophysical Research Communications
    Volume384
    Issue number2
    DOIs
    StatePublished - Jun 26 2009

    Keywords

    • Functionally important sites
    • Machine learning algorithms
    • Protein functional templates
    • Sparse kernel least squares classifiers

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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