Possible involvement of MAP kinase pathways in acquired metal-tolerance induced by heat in plants

Po Yu Chen, Kuo Ting Lee, Wen Chang Chi, Heribert Hirt, Ching Chun Chang*, Hao Jen Huang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Cross tolerance is a phenomenon that occurs when a plant, in resisting one form of stress, develops a tolerance to another form. Pretreatment with nonlethal heat shock has been known to protect cells from metal stress. In this study, we found that the treatment of rice roots with more than 25 μM of Cu2+ caused cell death. However, heat shock pretreatment attenuated Cu2+-induced cell death. The mechanisms of the cross tolerance phenomenon between heat shock and Cu2+ stress were investigated by pretreated rice roots with the protein synthesis inhibitor cycloheximide (CHX). CHX effectively block heat shock protection, suggesting that protection of Cu2+-induced cell death by heat shock was dependent on de novo protein synthesis. In addition, heat pretreatment downregulated ROS production and mitogen-activated protein kinase (MAPK) activities, both of which can be greatly elicited by Cu2+ stress in rice roots. Moreover, the addition of purified recombinant GST-OsHSP70 fusion proteins inhibited Cu 2+-enhanced MAPK activities in an in vitro kinase assay. Furthermore, loss of heat shock protection was observed in Arabidopsis mkk2 and mpk6 but not in mpk3 mutants under Cu2+ stress. Taken together, these results suggest that the interaction of OsHSP70 with MAPKs may contribute to the cellular protection in rice roots from excessive Cu2+ toxicity.

Original languageEnglish (US)
Pages (from-to)499-509
Number of pages11
JournalPlanta
Volume228
Issue number3
DOIs
StatePublished - Aug 1 2008

Keywords

  • Copper ion
  • Heat shock protein 70 (HSP70)
  • Metal stress
  • Mitogen-activated protein kinase (MAPK)

ASJC Scopus subject areas

  • Plant Science

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