Portable advanced off-axis camera for quantitative phase microscopy

Z. Monemhaghdoust, Christian Depeursinge, C. Moser

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We propose and experimentally demonstrate a device in which common-path interferometry combined with off-axis holographic geometry is used to realize a digital holographic camera which can be attached to the camera port of a conventional transmission microscope for complex wavefront analysis. A thick transmission volume grating recorded holographically into thick photosensitive glass splits the beam containing the sample information in two beams. The untouched transmitted beam creates the sample arm of the interferometer. The Bragg diffracted order of the grating is spectrally and spatially filtered by diffraction to generate a clean reference beam. Double passing the diffracted order through the grating using a retroreflector device provides filtering in two dimensions. The spatial filtering done by the grating which works based on high angular selectivity of thick volume gratings, reduces the alignment spatial sensitivity which is an advantage over the conventional spatial filtering done by pinholes. Besides, using a second thick grating, we introduce a desired coherence plane tilt in the reference beam which is sufficient to create high-visibility interference over the entire field of view. The full-field off-axis interferograms are created from which the amplitude and phase can be reconstructed. The advantage of the proposed camera is the insensitivity to the alignment, thus can be the basis for a standalone camera mountable on a standard optical microscope.

Original languageEnglish (US)
Title of host publicationThree-Dimensional and Multidimensional Microscopy
Subtitle of host publicationImage Acquisition and Processing XXI
PublisherSPIE
ISBN (Print)9780819498625
DOIs
StatePublished - Jan 1 2014
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI - San Francisco, CA, United States
Duration: Feb 3 2014Feb 6 2014

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8949
ISSN (Print)1605-7422

Other

OtherThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI
CountryUnited States
CitySan Francisco, CA
Period02/3/1402/6/14

Keywords

  • Diffraction gratings
  • Digital holographic microscopy
  • Off-axis full-field imaging
  • Phase microscopy
  • Portable camera

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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