Population genomic analysis of Aegilops tauschii identifies targets for bread wheat improvement

Kumar Gaurav, Sanu Arora, Paula Silva, Javier Sánchez-Martín, Richard Horsnell, Liangliang Gao, Gurcharn S. Brar, Victoria Widrig, W. John Raupp, Narinder Singh, Shuangye Wu, Sandip M. Kale, Catherine Chinoy, Paul Nicholson, Jesús Quiroz-Chávez, James Simmonds, Sadiye Hayta, Mark A. Smedley, Wendy Harwood, Suzannah PearceDavid Gilbert, Ngonidzashe Kangara, Catherine Gardener, Macarena Forner-Martínez, Jiaqian Liu, Guotai Yu, Scott A. Boden, Attilio Pascucci, Sreya Ghosh, Amber Hafeez, Tom O’Hara, Joshua Waites, Jitender Cheema, Burkhard Steuernagel, Mehran Patpour, Annemarie Fejer Justesen, Shuyu Liu, Jackie C. Rudd, Raz Avni, Amir Sharon, Barbara Steiner, Rizky Pasthika Kirana, Hermann Buerstmayr, Ali A. Mehrabi, Firuza Y. Nasyrova, Noam Chayut, Oadi Matny, Brian J. Steffenson, Nitika Sandhu, Parveen Chhuneja, Evans Lagudah, Ahmed F. Elkot, Simon Tyrrell, Xingdong Bian, Robert P. Davey, Martin Simonsen, Leif Schauser, Vijay K. Tiwari, H. Randy Kutcher, Pierre Hucl, Aili Li, Deng-Cai Liu, Long Mao, Steven Xu, Gina Brown-Guedira, Justin Faris, Jan Dvorak, Ming-Cheng Luo, Ksenia Krasileva, Thomas Lux, Susanne Artmeier, Klaus F. X. Mayer, Cristobal Uauy, Martin Mascher, Alison R. Bentley, Beat Keller, Jesse Poland, Brande B. H. Wulff

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Abstract$\textit{Aegilops tauschii}$, the diploid wild progenitor of the D subgenome of bread wheat, is a reservoir of genetic diversity for improving bread wheat performance and environmental resilience. Here we sequenced 242 $\textit{Ae. tauschii}$ accessions and compared them to the wheat D subgenome to characterize genomic diversity. We found that a rare lineage of $\textit{Ae. tauschii}$ geographically restricted to present-day Georgia contributed to the wheat D subgenome in the independent hybridizations that gave rise to modern bread wheat. Through $\textit{k}$-mer-based association mapping, we identified discrete genomic regions with candidate genes for disease and pest resistance and demonstrated their functional transfer into wheat by transgenesis and wide crossing, including the generation of a library of hexaploids incorporating diverse $\textit{Ae. tauschii}$ genomes. Exploiting the genomic diversity of the $\textit{Ae. tauschii}$ ancestral diploid genome permits rapid trait discovery and functional genetic validation in a hexaploid background amenable to breeding.
Original languageEnglish (US)
JournalNature Biotechnology
DOIs
StatePublished - Nov 1 2021

ASJC Scopus subject areas

  • Biomedical Engineering
  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Molecular Medicine
  • Biotechnology

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