Polymer-based monolithic microcolumns for hydrophobic interaction chromatography of proteins

Petrus Hemström, Anna Nordborg, Knut Irgum*, Frantisek Svec, Jean M.J. Fréchet

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

Monolithic capillary columns for hydrophobic interaction chromatography (HIC) have been prepared by thermally initiated, single-step in situ polymerization of mixtures of monovinyl monomers including butyl methacrylate and/or 2-hydroxyethyl methacrylate, with a divinyl crosslinker glycerol dimethacrylate or 1,4-butanediol dimethacrylate using two different porogen systems. Two porogenic solvent mixtures were used; one "hydrophilic", consisting of water, butanediol, and propanol, and one "hydrophobic," comprising dodecanol and cyclohexanol. The porous structures of the monoliths were characterized and their performance was demonstrated with a separation of a mixture of myoglobin, ribonuclease A, and lysozyme under conditions typical of HIC.

Original languageEnglish (US)
Pages (from-to)25-32
Number of pages8
JournalJournal of Separation Science
Volume29
Issue number1
DOIs
StatePublished - Jan 2006
Externally publishedYes

Keywords

  • Capillary
  • Column
  • HIC
  • Hydrophobic interaction chromatography
  • Monolith
  • Protein separation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Filtration and Separation

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