Plasma membrane Ca2+ channels in roots of higher plants and their role in aluminium toxicity

Miguel Piñeros*, Mark Tester

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Single Ca2+ channel records were obtained from plasma membrane-enriched fractions of wheat roots incorporated into artificial planar lipid bilayers. The channel had a unitary conductance of 15 pS for a 10 to 95 m M CaCl2 gradient (cytoplasm: outside of the cell). The voltage dependence displayed by the channel agreed with that expected for Ca2+ channels in the plasma membrane. The channel gating was strongly modified by addition of 20 μM extracellular verapamil (a Ca2+ channel antagonist). Extracellular AlCl3 (70 μM, pH 4.9) almost completely blocked the channel.

Original languageEnglish (US)
Pages (from-to)119-122
Number of pages4
JournalPlant and Soil
Volume155-156
Issue number1
DOIs
StatePublished - Oct 1 1993

Keywords

  • Ca channel
  • aluminium
  • plasma membrane
  • voltage clamp

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

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