Phosphorylation of histone H3 Ser10 establishes a hierarchy for subsequent intramolecular modification events

Stamatios Liokatis, Alexandra Stützer, Simon J. Elsässer, Francois Xavier Theillet, Rebecca Klingberg, Barth Van Rossum, Dirk Schwarzer, C. David Allis, Wolfgang Fischle, Philipp Selenko*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

Phosphorylation of Ser10 of histone H3 regulates chromosome condensation and transcriptional activity. Using time-resolved, high-resolution NMR spectroscopy, we demonstrate that histone H3 Ser10 phosphorylation inhibits checkpoint kinase 1 (Chk1)-and protein kinase C (PKC)-mediated modification of Thr11 and Thr6, the respective primary substrate sites of these kinases. On unmodified H3, both enzymes also target Ser10 and thereby establish autoinhibitory feedback states on individual H3 tails. Whereas phosphorylated Ser10 does not affect acetylation of Lys14 by Gcn5, phosphorylated Thr11 impedes acetylation. Our observations reveal mechanistic hierarchies of H3 phosphorylation and acetylation events and provide a framework for intramolecular modification cross-talk within the N terminus of histone H3.

Original languageEnglish (US)
Pages (from-to)819-823
Number of pages5
JournalNature Structural and Molecular Biology
Volume19
Issue number8
DOIs
StatePublished - Aug 1 2012

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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