Percoll and Ficoll self-generated density gradients by low-speed osmocentrifugation

Suzana Nunes*, Fernando Galembeck

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Percoll and Ficoll self-generated density gradients can be obtained by low-speed centrifugation of their solutions within dialysis cells. Useful Percoll density gradients can be obtained after 10-30 min centrifugation at 220-2010g, within dialysis cells. Ficoll density gradients, which are more difficult to self-generate, can be obtained by the same technique. Red cell band formation in a Percoll density gradient can be done in a single step by using dialysis cells as the centrifugation solution container.

Original languageEnglish (US)
Pages (from-to)48-51
Number of pages4
JournalAnalytical Biochemistry
Volume146
Issue number1
DOIs
StatePublished - Jan 1 1985

Keywords

  • Ficoll
  • Percoll
  • cell fractionation
  • density gradients
  • osmocentrifugation
  • self-generated density gradients

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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