Ozone and nitric oxide induce cGMP-dependent and -independent transcription of defence genes in tobacco

Stefania Pasqualini*, Stuart Meier, Chris Gehring, Laura Madeo, Marco Fornaciari, Bruno Romano, Luisa Ederli

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

81 Scopus citations

Abstract

• Here, we analyse the temporal signatures of ozone (O 3)-induced hydrogen peroxide (H2O2) and nitric oxide (NO) and the role of the second messenger guanosine 3′,5′- cyclic monophosphate (cGMP) in transcriptional changes of genes diagnostic for biotic and abiotic stress responses. • Within 90 min O3 induced H2O2 and NO peaks and we demonstrate that NO donors cause rapid H2O2 accumulation in tobacco (Nicotiana tabacum) leaf. Ozone also causes highly significant, late (> 2 h) and sustained cGMP increases, suggesting that the second messenger may not be required in all early (< 2 h) responses to O3, but is essential and sufficient for the induction of some O3-dependent pathways. • This hypothesis was tested resolving the time course of O3-induced transcript accumulation of alternative oxidase (AOX1a), glutathione peroxidase (GPX), aminocyclopropancarboxylic acid synthase (ACS2) that is critical for the synthesis of ethylene, phenylalanine ammonia lyase (PALa) and the pathogenesis-related protein PR1a. • The data show that early O3 and NO caused transcriptional activation of the scavenger encoding proteins AOX1a, GPX and the induction of ethylene production through ACS2 are cGMP independent. By contrast, the early response of PALa and the late response of PR1a show critical dependence on cGMP.

Original languageEnglish (US)
Pages (from-to)860-870
Number of pages11
JournalNew Phytologist
Volume181
Issue number4
DOIs
StatePublished - Mar 1 2009

Keywords

  • Defence gene induction
  • Hydrogen peroxide (HO)
  • Nicotiana tabacum (tobacco)
  • Nitric oxide (NO)
  • Ozone
  • cGMP

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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