OXI1 kinase is necessary for oxidative burst-mediated signalling in Arabidopsis

Malke C. Rentel*, David Lecourieux, Fatma Ouaked, Sarah L. Usher, Lindsay Petersen, Haruko Okamoto, Heather Knight, Scott C. Peck, Claire S. Grierson, Heribert Hirt, Marc R. Knight

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

403 Scopus citations

Abstract

Active oxygen species (AOS) generated in response to stimuli and during development can function as signalling molecules in eukaryotes, leading to specific downstream responses. In plants these include such diverse processes as coping with stress (for example pathogen attack, wounding and oxygen deprivation), abscisic-acid-induced guard-cell closure, and cellular development (for example root hair growth). Despite the importance of signalling via AOS in eukaryotes, little is known about the protein components operating downstream of AOS that mediate any of these processes. Here we show that expression of an Arabidopsis thaliana gene (OXI1) encoding a serine/threonine kinase is induced in response to a wide range of H 2O2-generating stimuli. OXI1 kinase activity is itself also induced by H2O2 in vivo. OXI1 is required for full activation of the mitogen-activated protein kinases (MAPKs) MPK3 and MPK6 after treatment with AOS or elicitor and is necessary for at least two very different AOS-mediated processes: basal resistance to Peronospora parasitica infection, and root hair growth. Thus, OXI1 is an essential part of the signal transduction pathway linking oxidative burst signals to diverse downstream responses.

Original languageEnglish (US)
Pages (from-to)858-861
Number of pages4
JournalNature
Volume427
Issue number6977
DOIs
StatePublished - Feb 26 2004

ASJC Scopus subject areas

  • General

Fingerprint

Dive into the research topics of 'OXI1 kinase is necessary for oxidative burst-mediated signalling in Arabidopsis'. Together they form a unique fingerprint.

Cite this