Optical tweezers for sample fixing in micro-diffraction experiments

H. Amenitsch*, D. Cojoc, M. Rappolt, B. Sartori, P. Laggner, E. Ferrari, V. Garbin, M. Burghammer, Ch Riekel, E. Di Fabrizio

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

In order to manipulate, characterize and measure the micro-diffraction of individual structural elements down to single phospholipid liposomes we have been using optical tweezers (OT) combined with an imaging microscope. We were able to install the OT system at the microfocus beamline ID13 at the ESRF and trap clusters of about 50 multi-lamellar liposomes (< 10 μm large cluster). Further we have performed a scanning diffraction experiment with a 1 micrometer beam to demonstrate the fixing capabilities and to confirm the size of the liposome cluster by X-ray diffraction.

Original languageEnglish (US)
Title of host publicationSYNCHROTRON RADIATION INSTRUMENTATION
Subtitle of host publicationNinth International Conference on Synchrotron Radiation Instrumentation
Pages1287-1290
Number of pages4
Volume879
DOIs
StatePublished - 2007
Externally publishedYes
EventSYNCHROTRON RADIATION INSTRUMENTATION: 9th International Conference on Synchrotron Radiation Instrumentation - Daegu, Korea, Republic of
Duration: May 28 2006Jun 28 2006

Other

OtherSYNCHROTRON RADIATION INSTRUMENTATION: 9th International Conference on Synchrotron Radiation Instrumentation
CountryKorea, Republic of
CityDaegu
Period05/28/0606/28/06

Keywords

  • Optical manipulation
  • Phospholipids
  • Single biological entity
  • X-ray micro diffraction

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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