Open-tubular capillary columns with a porous layer of monolithic polymer for highly efficient and fast separations in electrochromatography

Sebastiaan Eeltink, Frantisek Svec, Jean Frechet*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

76 Scopus citations

Abstract

Open-tubular columns for CEC separations having inner-wall coated with a thin layer of porous monolithic polymer have been studied. A two-step process including (i) UV-initiated polymerization leading to a layer of porous poly(butyl methacrylate-co-ethylene dimethacrylate), and (ii) UV-initiated grafting of ionizable monomers appear to be well suited for the preparation of these columns. The thickness of the porous polymer layer is controlled by the percentage of monomers in the polymerization mixture and/or length of the irradiation time. The layer thickness significantly affects retention, efficiency, and resolution in open-tubular CEC. Under optimized conditions, column efficiencies up to 400 000 plates/m can be achieved. Use of higher temperature and application of pressure enables a significant acceleration of the open-tubular CEC separations.

Original languageEnglish (US)
Pages (from-to)4249-4256
Number of pages8
JournalElectrophoresis
Volume27
Issue number21
DOIs
StatePublished - Nov 1 2006

Keywords

  • CEC
  • Capillary column porous-layer
  • External band broadening
  • Monolith
  • Open-tubular column

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

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