On the use of denaturing gradient gel electrophoresis approach for bacterial identification in endodontic infections

Julio C. Machado de Oliveira, Tulio G.V. Gama, José F. Siqueira, Isabela N. Rôças, Raquel S. Peixoto, Alexandre S. Rosado

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Bacteria in infected root canals of teeth evincing chronic apical periodontitis lesions were identified by a polymerase chain reaction - denaturing gradient gel electrophoresis (PCR-DGGE) approach. DNA was extracted from root canal samples, and part of the 16S rRNA gene of all bacteria was amplified by PCR and separated by DGGE, generating banding patterns representative of the community structure. Twenty visible bands were cut out of the gel, re-amplified, and sequenced to provide identification. Sequencing analysis revealed the presence of both cultivable and as-yet-uncultivated species in the samples analyzed, including representatives of the genera Fusobacterium, Bacteroides, Dialister, Synergistes, Prevotella, Eubacterium and Peptostreptococcus. Unambiguous identification was not always possible and the method's limitations are discussed. In general, the findings showed that PCR-DGGE can be useful for the identification of both cultivable and as-yet-uncultivated bacteria in endodontic infections. © Springer-Verlag 2006.
Original languageEnglish (US)
Pages (from-to)127-132
Number of pages6
JournalClinical Oral Investigations
Volume11
Issue number2
DOIs
StatePublished - Jun 1 2007
Externally publishedYes

Fingerprint

Dive into the research topics of 'On the use of denaturing gradient gel electrophoresis approach for bacterial identification in endodontic infections'. Together they form a unique fingerprint.

Cite this