On the substrate- and stereospecificity of the plant carotenoid cleavage dioxygenase 7

Mark Bruno, Manuel Hofmann, Martina Vermathen, Adrian Alder, Peter D. Beyer, Salim Al-Babili

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Strigolactones are phytohormones synthesized from carotenoids via a stereospecific pathway involving the carotenoid cleavage dioxygenases 7 (CCD7) and 8. CCD7 cleaves 9-cis-β-carotene to form a supposedly 9-cis-configured β-apo-10′-carotenal. CCD8 converts this intermediate through a combination of yet undetermined reactions into the strigolactone-like compound carlactone. Here, we investigated the substrate and stereo-specificity of the Arabidopsis and pea CCD7 and determined the stereo-configuration of the β-apo-10′-carotenal intermediate by using Nuclear Magnetic Resonance Spectroscopy. Our data unequivocally demonstrate the 9-cis-configuration of the intermediate. Both CCD7s cleave different 9-cis-carotenoids, yielding hydroxylated 9-cis-apo-10′-carotenals that may lead to hydroxylated carlactones, but show highest affinity for 9-cis-β-carotene. © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Original languageEnglish (US)
Pages (from-to)1802-1807
Number of pages6
JournalFEBS Letters
Volume588
Issue number9
DOIs
StatePublished - Mar 28 2014

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Biophysics

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