Off-axis digital holographic camera for quantitative phase microscopy

Zahra Monemhaghdoust, Frédéric Montfort, Yves Emery, Christian Depeursinge, Christophe Moser

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

We propose and experimentally demonstrate a digital holographic camera which can be attached to the camera port of a conventional microscope for obtaining digital holograms in a self-reference configuration, under short coherence illumination and in a single shot. A thick holographic grating filters the beam containing the sample information in two dimensions through diffraction. The filtered beam creates the reference arm of the interferometer. The spatial filtering method, based on the high angular selectivity of the thick grating, reduces the alignment sensitivity to angular displacements compared with pinhole based Fourier filtering. The addition of a thin holographic grating alters the coherence plane tilt introduced by the thick grating so as to create highvisibility interference over the entire field of view. The acquired full-field off-axis holograms are processed to retrieve the amplitude and phase information of the sample. The system produces phase images of cheek cells qualitatively similar to phase images extracted with a standard commercial DHM.

Original languageEnglish (US)
Pages (from-to)1721-1730
Number of pages10
JournalBiomedical Optics Express
Volume5
Issue number6
DOIs
StatePublished - Jun 1 2014

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biotechnology

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