Novel lncRNAs in myogenesis: a miR-31 overlapping transcript controls myoblast differentiation.

Monica Ballarino, Valentina Cazzella, Daniel D'Andrea, Luigi Grassi, Lavinia Bisceglie, Andrea Cipriano, Tiziana Santini, Chiara Pinnarò, Mariangela Morlando, Anna Tramontano, Irene Bozzoni

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

Transcriptome analysis allowed the identification of new long noncoding RNAs differentially expressed during murine myoblast differentiation. These transcripts were classified on the basis of their expression under proliferating versus differentiated conditions, muscle-restricted activation, and subcellular localization. Several species displayed preferential expression in dystrophic (mdx) versus wild-type muscles, indicating their possible link with regenerative processes. One of the identified transcripts, lnc-31, even if originating from the same nuclear precursor of miR-31, is produced by a pathway mutually exclusive. We show that lnc-31 and its human homologue hsa-lnc-31 are expressed in proliferating myoblasts, where they counteract differentiation. In line with this, both species are more abundant in mdx muscles and in human Duchenne muscular dystrophy (DMD) myoblasts, than in their normal counterparts. Altogether, these data suggest a crucial role for lnc-31 in controlling the differentiation commitment of precursor myoblasts and indicate that its function is maintained in evolution despite the poor sequence conservation with the human counterpart.
Original languageEnglish (US)
Pages (from-to)728-736
Number of pages9
JournalMolecular and Cellular Biology
Volume35
Issue number4
DOIs
StatePublished - Dec 15 2014
Externally publishedYes

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