The entorhinal cortex and the amygdala are interconnected structures of the limbic system in which paroxysmal activity occurs during temporal lobe epilepsy. Conflicting evidence shows that noradrenaline (i) inhibits the spreading to other parts of the limbic system of paroxysmal activity generated in the amygdala or the entorhinal cortex, but also (ii) increases glutamatergic transmission in the basolateral amygdala. Given our previous work on the inhibitory effect of noradrenaline on entorhinal cortex neurons, we developed an in vitro slice preparation to study the synaptic transmission in the basolateral amygdala and its modulation by noradrenaline. Noradrenaline reduced the fast excitatory postsynaptic potential (EPSP) by ~ 40% at 100 μM and the slow EPSP by ~ 50% at 50 μM. A similar effect was obtained with the α2-agonist UK 14304 at 100 and 50 μM respectively. In contrast, the β-agonist isoproterenol increased the fast EPSP by ~ 40% at 100 μM and the slow EPSP by ~ 20% at 50 μM. Accordingly, the effect of noradrenaline on the EPSPs was blocked by the α2-antagonist yohimbine (10 μM) but not by the α1-antagonist prazosine (10 μM) and the β-antagonist propranolol (10 μM). Noradrenaline (50-100 μM) was ineffective on most (14/16) of the isolated inhibitory postsynaptic potentials (IPSPs). These experiments provide evidence that noradrenaline inhibits the excitatory synaptic response of basolateral amygdala neurons. A pharmacological analysis revealed that the noradrenergic modulation of the excitatory transmission in the basolateral amygdala can be dissected into a predominant α2-adrenoreceptor-mediated inhibition and a β-adrenoreceptor-mediated excitation.
|Original language||English (US)|
|Number of pages||9|
|Journal||European Journal of Neuroscience|
|State||Published - Aug 2 1997|
- Basolateral amygdala
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