Noradrenaline modulates glutamate-mediated neurotransmission in the rat basolateral amygdala in vitro

Barbara Ferry, Pierre Magistretti, Etienne Pralong*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

85 Scopus citations

Abstract

The entorhinal cortex and the amygdala are interconnected structures of the limbic system in which paroxysmal activity occurs during temporal lobe epilepsy. Conflicting evidence shows that noradrenaline (i) inhibits the spreading to other parts of the limbic system of paroxysmal activity generated in the amygdala or the entorhinal cortex, but also (ii) increases glutamatergic transmission in the basolateral amygdala. Given our previous work on the inhibitory effect of noradrenaline on entorhinal cortex neurons, we developed an in vitro slice preparation to study the synaptic transmission in the basolateral amygdala and its modulation by noradrenaline. Noradrenaline reduced the fast excitatory postsynaptic potential (EPSP) by ~ 40% at 100 μM and the slow EPSP by ~ 50% at 50 μM. A similar effect was obtained with the α2-agonist UK 14304 at 100 and 50 μM respectively. In contrast, the β-agonist isoproterenol increased the fast EPSP by ~ 40% at 100 μM and the slow EPSP by ~ 20% at 50 μM. Accordingly, the effect of noradrenaline on the EPSPs was blocked by the α2-antagonist yohimbine (10 μM) but not by the α1-antagonist prazosine (10 μM) and the β-antagonist propranolol (10 μM). Noradrenaline (50-100 μM) was ineffective on most (14/16) of the isolated inhibitory postsynaptic potentials (IPSPs). These experiments provide evidence that noradrenaline inhibits the excitatory synaptic response of basolateral amygdala neurons. A pharmacological analysis revealed that the noradrenergic modulation of the excitatory transmission in the basolateral amygdala can be dissected into a predominant α2-adrenoreceptor-mediated inhibition and a β-adrenoreceptor-mediated excitation.

Original languageEnglish (US)
Pages (from-to)1356-1364
Number of pages9
JournalEuropean Journal of Neuroscience
Volume9
Issue number7
DOIs
StatePublished - Aug 2 1997

Keywords

  • Basolateral amygdala
  • Epilepsy
  • Glutamate
  • Memory

ASJC Scopus subject areas

  • Neuroscience(all)

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