Noninvasive microelectrode ion flux estimation technique (MIFE) for the study of the regulation of root membrane transport by cyclic nucleotides

Natalia Maria Ordoñez, Lana Shabala, Christoph A Gehring, Sergey Nikolayevich Shabala

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Changes in ion permeability and subsequently intracellular ion concentrations play a crucial role in intracellular and intercellular communication and, as such, confer a broad array of developmental and adaptive responses in plants. These changes are mediated by the activity of plasma-membrane based transport proteins many of which are controlled by cyclic nucleotides and/or other signaling molecules. The MIFE technique for noninvasive microelectrode ion flux measuring allows concurrent quantification of net fluxes of several ions with high spatial (μm range) and temporal (ca. 5 s) resolution, making it a powerful tool to study various aspects of downstream signaling events in plant cells. This chapter details basic protocols enabling the application of the MIFE technique to study regulation of root membrane transport in general and cyclic nucleotide mediated transport in particular. © Springer Science+Business Media New York 2013.
Original languageEnglish (US)
Pages (from-to)95-106
Number of pages12
JournalMethods in Molecular Biology
Volume1016
DOIs
StatePublished - Sep 3 2013

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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