Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy

Benjamin Rappaz*, Elena Cano, Tristan Colomb, Jonas Kühn, Christian Depeursinge, Viesturs Simanis, Pierre Magistretti, Pierre Marquet

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

156 Scopus citations

Abstract

Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, including dry mass production, density, and spatial distribution. We have applied DHM to study the dry mass production rate and the dry mass surface density in wild-type and mutant fission yeast cells. Our study demonstrates the applicability of DHM as a tool for label-free quantitative analysis of the cell cycle and opens the possibility for its use in high-throughput screening.

Original languageEnglish (US)
Article number034049
JournalJournal of Biomedical Optics
Volume14
Issue number3
DOIs
StatePublished - Dec 1 2009

Keywords

  • cell cycle
  • cell growth
  • digital holographic microscopy
  • dry mass
  • fission yeast
  • quantitative phase image

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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