Non-invasive dry mass determination and monitoring at the single cell level with digital holographic microscopy

Benjamin Rappaz*, Elena Cano, Jonas Kühn, Tristan Colomb, Christian Depeursinge, Pierre J. Magistretti, Pierre Marquet

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Digital holography microscopy (DHM) is an optical microscopy technique which allows recording non-invasively the phase shift induced by living cells with nanometric sensitivity. Here, we exploit the phase signal as an indicator of dry mass (related to the protein concentration). This parameter allows monitoring the protein production rate and its evolution during the cell cycle.

Original languageEnglish (US)
Title of host publicationBiophotonics
Subtitle of host publicationPhotonic Solutions for Better Health Care
Volume6991
DOIs
StatePublished - 2008
Externally publishedYes
EventBiophotonics: Photonic Solutions for Better Health Care - Strasbourg, France
Duration: Apr 8 2008Apr 10 2008

Other

OtherBiophotonics: Photonic Solutions for Better Health Care
CountryFrance
CityStrasbourg
Period04/8/0804/10/08

Keywords

  • Cell cycle
  • Cell growth
  • Digital holography
  • Dry mass
  • Fission yeast
  • Protein production
  • Quantitative phase microscopy
  • Schizosaccharomyces pombe

ASJC Scopus subject areas

  • Engineering(all)

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