NO release by nitric oxide donors in vitro and in planta

Luisa Ederli, Lara Reale, Laura Madeo, Francesco Ferranti, Christoph Gehring, Marco Fornaciari, Bruno Romano, Stefania Pasqualini*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

73 Scopus citations

Abstract

Artificial nitric oxide (NO) donors are widely used as tools to study the role of NO in plants. However, reliable and reproducible characterisations of metabolic responses induced by different NO donors is complicated by the variability of their NO release characteristics. The latter are affected by different physical and biological factors including temperature and light. Here we critically evaluate NO release characteristics of the donors sodium nitroprusside (SNP), S-nitrosoglutathione (GSNO) and nitric oxide synthase (NOS), both in vitro and in planta (Nicotiana tabacum L. cv. BelW3) and assess their effects on NO dependent processes such as the transcriptional regulation of the mitochondrial alternative oxidase gene (AOX1a), accumulation of H2O2 and induction of cell death. We demonstrate that, contrary to NOS and SNP, GSNO is not an efficient NO generator in leaf tissue. Furthermore, spectrophotometric measurement of NO with a haemoglobin assay, rather than diaminofluorescein (DAF-FM) based detection, is best suited for the quantification of tissue NO. In spite of the different NO release signatures by SNP and NOS in tissue, the NO dependent responses examined were similar, suggesting that there is a critical threshold for the NO response.

Original languageEnglish (US)
Pages (from-to)42-48
Number of pages7
JournalPlant Physiology and Biochemistry
Volume47
Issue number1
DOIs
StatePublished - Jan 1 2009

Keywords

  • Alternative oxidase
  • Cell death
  • NOS
  • Nitric oxide
  • SNP
  • Tobacco

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

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