Nitrilotriacetic Acid-Modified Magnetic Nanoparticles as a General Agent to Bind Histidine-Tagged Proteins

Chenjie Xu, Keming Xu, Hongwei Gu, Xiaofen Zhong, Zhihong Guo, Rongkun Zheng, Xixiang Zhang, Bing Xu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

449 Scopus citations

Abstract

Using Nα,Nα-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt?NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins.

Original languageEnglish (US)
Pages (from-to)3392-3393
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number11
DOIs
StatePublished - Mar 22 2004

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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