Neuroprotective role of lactate after cerebral ischemia

Carole Berthet, Hongxia Lei, Jonathan Thevenet, Rolf Gruetter, Pierre Magistretti, Lorenz Hirt*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

145 Scopus citations

Abstract

It is well established that lactate can be used as an energy substrate by the brain by conversion to pyruvate and a subsequent oxidation in the mitochondria. Knowing the need for readily metabolizable substrates directly after ischemia and the protective effect of lactate after excitotoxicity, the aim of this study was to investigate whether lactate administration directly after ischemia could be neuroprotective. In vitro, the addition of 4 mmol/L L-lactate to the medium of rat organotypic hippocampal slices, directly after oxygen and glucose deprivation (OGD), protected against neuronal death, whereas a higher dose of 20 mmol/L was toxic. In vivo, after middle cerebral artery occlusion in the mouse, an intracerebroventricular injection of 2 μL of 100 mmol/L L-lactate, immediately after reperfusion, led to a significant decrease in lesion size, which was more pronounced in the striatum, and an improvement in neurologic outcome. A later injection 1 h after reperfusion did not reduce lesion size, but significantly improved neurologic outcome, which is an important point in the context of a potential clinical application. Therefore, a moderate increase in lactate after ischemia may be a therapeutic tool.

Original languageEnglish (US)
Pages (from-to)1780-1789
Number of pages10
JournalJournal of Cerebral Blood Flow and Metabolism
Volume29
Issue number11
DOIs
StatePublished - Jul 15 2009

Keywords

  • L-lactate
  • Magnetic resonance spectroscopy
  • Middle cerebral artery occlusion
  • Oxygen and glucose deprivation

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

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