Nef-induced major histocompatibility complex class I downregulation is functionally dissociated from its virion incorporation, enhancement of viral infectivity, and CD4 down-regulation

Hirofumi Akari*, Stefan Arold, Tomoharu Fukumori, Toshiyuki Okazaki, Klaus Strebel, Akio Adachi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

96 Scopus citations

Abstract

The N-terminal alpha-helix domain of the human immunodeficiency virus type 1 (HIV-1) Nef protein plays important roles in enhancement of viral infectivity, virion incorporation of Nef, and the down-regulation of major histocompatibility complex class I (MHC-I) expression on cell surfaces. In this study, we demonstrated that Met 20 in the alpha-helix domain was indispensable for the ability of Nef to modulate MHC-I expression but not for other events. We also showed that Met 20 was unnecessary for the down- regulation of CD4. These findings indicate that the region governing MHC-I down-regulation is proximate in the alpha-helix domain but is dissociated functionally from that determining enhancement of viral infectivity, virion incorporation of Nef, and CD4 down-regulation.

Original languageEnglish (US)
Pages (from-to)2907-2912
Number of pages6
JournalJournal of Virology
Volume74
Issue number6
DOIs
StatePublished - Mar 8 2000

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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